Results from both setups showed similar yield and purity as what can be expected from a traditional setup performed in batch runs. HiScreenCapto S ImpAct HiTrap Capto S ImpAct, 1 ml, 5 ml Prepacked column Instructions for Use Capto S ImpAct is a strong cation exchange BioProcess chromatography medium (resin) for intermediate purification and polishing of a wide range of biomolecules, especially monoclonal antibodies. High binding capacity, > 100 mg MAb/mL medium. ![]() The medium is designed for polishing of monoclonal antibodies (MAbs) and a wide range of other biomolecules. Capto S ImpAct is a strong cation exchange chromatography medium (resin) developed for intermediate purification and polishing of monoclonal antibodies (MAbs) and other biomolecules. The second Capto adhere step was performed in FT mode in both setups. Capto S ImpAct Capto S ImpAct chromatography medium (resin) is a strong cation exchanger (Fig 1). Two setups were evaluated, where the first Capto S ImpAct polishing step was run in either bind-elute (B/E) or flow-through (FT) mode. Capto™ S ImpAct and Capto adhere media in serially connected columns with an intermediate in-line conditioning step were used. The capture step was followed by two polishing steps in an STP setup on the ÄKTA pure chromatography system. ![]() Using the 3C PCC setup, the capacity utilization could be increased by 56% as compared with an equivalent batch run. The results showed consistent yield and purity over time. To assess robustness of the setup, 10 cycles were performed. Capto MMC and Nuvia cPrime, in a range of solvents, a Lennard-Jones. MAb capture was performed using MabSelect SuRe™ LX protein A chromatography medium (resin) in a three-column PCC (3C PCC) setup on the ÄKTA™ pcc 75 chromatography system. ![]() In this study, periodic counter current (PCC) chromatography and straight-through processing (STP) technologies were evaluated in a continuous three-step monoclonal antibody (MAb) purification process.
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